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s6 ribosomal protein  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc s6 ribosomal protein
    Rapamycin co‐treatment mitigates proinflammatory transcriptional hyperactivation in aged mice exposed to HFD. (A) Protocol: Mice received ND or eRapamycin‐containing diet from 4 to 18 months, followed by 9 weeks on HFD or ND. (B) Western blot of liver tissue. (C) Fold change of <t>p‐S6/S6</t> compared to control diet. (D) PCA of RNA‐seq from isolated hepatocytes. (E) Number of DEGs across treatment groups. (F) Scatter plot comparing HFD‐induced gene expression changes (HFD + veh vs. ND + veh) to all gene expression changes with eRapamycin (eRapa+HFD vs. HFD + veh). (G) Heatmap of genes upregulated by HFD + vehicle treatment. (H) Venn diagram showing overlap between transcripts elevated by HFD versus ND and those decreased by rapamycin. (I) KEGG enrichment analysis of overlapping genes. (J) Heatmap of innate immune response genes. (K) Top transcription factor pathways predicted by IPA in the overlapping gene set. (L) Heatmap of Stat1 target genes. (M) Tumorigenic index score calculated per mouse. Statistical analyses for p‐S6/S6 ratios and tumorigenic index scores were performed using one‐way ANOVA with post hoc Tukey's test; p < 0.05 (*), p < 0.1 (**).
    S6 Ribosomal Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 4737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stat1+protein/Stat1+Antibody/pmc12882742-179-8-11
    Average 97 stars, based on 4737 article reviews
    s6 ribosomal protein - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "Rapamycin Reverses the Hepatic Response to Diet‐Induced Metabolic Stress That Is Amplified by Aging"

    Article Title: Rapamycin Reverses the Hepatic Response to Diet‐Induced Metabolic Stress That Is Amplified by Aging

    Journal: Aging Cell

    doi: 10.1111/acel.70395

    Rapamycin co‐treatment mitigates proinflammatory transcriptional hyperactivation in aged mice exposed to HFD. (A) Protocol: Mice received ND or eRapamycin‐containing diet from 4 to 18 months, followed by 9 weeks on HFD or ND. (B) Western blot of liver tissue. (C) Fold change of p‐S6/S6 compared to control diet. (D) PCA of RNA‐seq from isolated hepatocytes. (E) Number of DEGs across treatment groups. (F) Scatter plot comparing HFD‐induced gene expression changes (HFD + veh vs. ND + veh) to all gene expression changes with eRapamycin (eRapa+HFD vs. HFD + veh). (G) Heatmap of genes upregulated by HFD + vehicle treatment. (H) Venn diagram showing overlap between transcripts elevated by HFD versus ND and those decreased by rapamycin. (I) KEGG enrichment analysis of overlapping genes. (J) Heatmap of innate immune response genes. (K) Top transcription factor pathways predicted by IPA in the overlapping gene set. (L) Heatmap of Stat1 target genes. (M) Tumorigenic index score calculated per mouse. Statistical analyses for p‐S6/S6 ratios and tumorigenic index scores were performed using one‐way ANOVA with post hoc Tukey's test; p < 0.05 (*), p < 0.1 (**).
    Figure Legend Snippet: Rapamycin co‐treatment mitigates proinflammatory transcriptional hyperactivation in aged mice exposed to HFD. (A) Protocol: Mice received ND or eRapamycin‐containing diet from 4 to 18 months, followed by 9 weeks on HFD or ND. (B) Western blot of liver tissue. (C) Fold change of p‐S6/S6 compared to control diet. (D) PCA of RNA‐seq from isolated hepatocytes. (E) Number of DEGs across treatment groups. (F) Scatter plot comparing HFD‐induced gene expression changes (HFD + veh vs. ND + veh) to all gene expression changes with eRapamycin (eRapa+HFD vs. HFD + veh). (G) Heatmap of genes upregulated by HFD + vehicle treatment. (H) Venn diagram showing overlap between transcripts elevated by HFD versus ND and those decreased by rapamycin. (I) KEGG enrichment analysis of overlapping genes. (J) Heatmap of innate immune response genes. (K) Top transcription factor pathways predicted by IPA in the overlapping gene set. (L) Heatmap of Stat1 target genes. (M) Tumorigenic index score calculated per mouse. Statistical analyses for p‐S6/S6 ratios and tumorigenic index scores were performed using one‐way ANOVA with post hoc Tukey's test; p < 0.05 (*), p < 0.1 (**).

    Techniques Used: Western Blot, Control, RNA Sequencing, Isolation, Gene Expression

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    Article Title: The good and the bad faces of STAT1 in solid tumours.
    Article Snippet: Lee, STAT1 as a key modulator of cell death, Cell Signal 19 (2007) 454–465.

    Article Title: Interferon activates promoter of Nmi gene via interferon regulator factor-1.
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    Article Title: Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo
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    Article Title: Phosphatase wild-type p53-induced phosphatase 1 controls the development of T H 9 cells and allergic airway inflammation.
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    Article Title: Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo
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    Article Title: Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.
    Article Snippet: This is a PDF file of an unedited peer-reviewed manuscript that has been accepted for publication.. NPG are providing this early version of the manuscript as a service to our customers.. The manuscript will undergo copyediting, typesetting and a proof review before it is published in its final form.

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    Article Title: The good and the bad faces of STAT1 in solid tumours.
    Article Snippet: Lee, STAT1 as a key modulator of cell death, Cell Signal 19 (2007) 454–465.

    Article Title: Interferon activates promoter of Nmi gene via interferon regulator factor-1.
    Article Snippet: N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses.. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated.. We identified and cloned the promoter of Nmi gene.

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    Article Title: Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo
    Article Snippet: The phosphorylation status of STAT1 in cancer cells was assessed by treating the cells with 1,000 U of IFN-b for 24 h. The cell lysate collected from IFN-b-challenged and control-media-treated cells were subjected to immunoblotting to assess the levels of STAT1 (cat. no. 14994) and p-STAT1 (Tyr701, cat. no. 9167S) (Cell Signaling Technology).

    Article Title: Phosphatase wild-type p53-induced phosphatase 1 controls the development of T H 9 cells and allergic airway inflammation.
    Article Snippet: Phospho-STAT6 (Tyr641), STAT6, phospho-Smad3 319 (Ser423/425), Smad3, phospho-p38 MAPK (Thr180/Tyr182), P38, p-STAT5 320 (Tyr694), STAT5, p-STAT1 (Ser727), STAT1, phospho-SAPK/JNK 321 (Thr183/Tyr185), SAPK/JNK, phospho-c-Jun (Ser73), c-Jun, Rel-B, NF-κB p65, 322 β-Actin and histone H3 were purchased from CST company.

    Article Title: Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.
    Article Snippet: This is a PDF file of an unedited peer-reviewed manuscript that has been accepted for publication.. NPG are providing this early version of the manuscript as a service to our customers.. The manuscript will undergo copyediting, typesetting and a proof review before it is published in its final form.

    Article Title: Role of MSC-derived Galectin 3 in the AML Microenvironment
    Article Snippet: STAT1 , stat1 , Cell Signaling , 9172 , Rabbit , 250.

    Article Title: Antagonism between viral infection and innate immunity at the single-cell level
    Article Snippet: STAT1 was reported to promote cell death [Kim & Lee, 2007, Cell Signal., doi:10.1016/j.cellsig.2006.09.003], so inhibition of STAT activity in infected cells is expected to promote virus spread.

    Article Title: The good and the bad faces of STAT1 in solid tumours.
    Article Snippet: Lee, STAT1 as a key modulator of cell death, Cell Signal 19 (2007) 454–465.

    Article Title: Interferon activates promoter of Nmi gene via interferon regulator factor-1.
    Article Snippet: N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses.. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated.. We identified and cloned the promoter of Nmi gene.

    Bicinchoninic Acid Protein Assay:


    Article Title: Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo
    Article Snippet: The phosphorylation status of STAT1 in cancer cells was assessed by treating the cells with 1,000 U of IFN-b for 24 h. The cell lysate collected from IFN-b-challenged and control-media-treated cells were subjected to immunoblotting to assess the levels of STAT1 (cat. no. 14994) and p-STAT1 (Tyr701, cat. no. 9167S) (Cell Signaling Technology).

    Article Title: Phosphatase wild-type p53-induced phosphatase 1 controls the development of T H 9 cells and allergic airway inflammation.
    Article Snippet: Phospho-STAT6 (Tyr641), STAT6, phospho-Smad3 319 (Ser423/425), Smad3, phospho-p38 MAPK (Thr180/Tyr182), P38, p-STAT5 320 (Tyr694), STAT5, p-STAT1 (Ser727), STAT1, phospho-SAPK/JNK 321 (Thr183/Tyr185), SAPK/JNK, phospho-c-Jun (Ser73), c-Jun, Rel-B, NF-κB p65, 322 β-Actin and histone H3 were purchased from CST company.

    Article Title: Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.
    Article Snippet: This is a PDF file of an unedited peer-reviewed manuscript that has been accepted for publication.. NPG are providing this early version of the manuscript as a service to our customers.. The manuscript will undergo copyediting, typesetting and a proof review before it is published in its final form.

    Article Title: Role of MSC-derived Galectin 3 in the AML Microenvironment
    Article Snippet: STAT1 , stat1 , Cell Signaling , 9172 , Rabbit , 250.

    Article Title: Antagonism between viral infection and innate immunity at the single-cell level
    Article Snippet: STAT1 was reported to promote cell death [Kim & Lee, 2007, Cell Signal., doi:10.1016/j.cellsig.2006.09.003], so inhibition of STAT activity in infected cells is expected to promote virus spread.

    Article Title: The good and the bad faces of STAT1 in solid tumours.
    Article Snippet: Lee, STAT1 as a key modulator of cell death, Cell Signal 19 (2007) 454–465.

    Article Title: Interferon activates promoter of Nmi gene via interferon regulator factor-1.
    Article Snippet: N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses.. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated.. We identified and cloned the promoter of Nmi gene.

    AST Assay:


    Article Title: Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo
    Article Snippet: The phosphorylation status of STAT1 in cancer cells was assessed by treating the cells with 1,000 U of IFN-b for 24 h. The cell lysate collected from IFN-b-challenged and control-media-treated cells were subjected to immunoblotting to assess the levels of STAT1 (cat. no. 14994) and p-STAT1 (Tyr701, cat. no. 9167S) (Cell Signaling Technology).

    Article Title: Phosphatase wild-type p53-induced phosphatase 1 controls the development of T H 9 cells and allergic airway inflammation.
    Article Snippet: Phospho-STAT6 (Tyr641), STAT6, phospho-Smad3 319 (Ser423/425), Smad3, phospho-p38 MAPK (Thr180/Tyr182), P38, p-STAT5 320 (Tyr694), STAT5, p-STAT1 (Ser727), STAT1, phospho-SAPK/JNK 321 (Thr183/Tyr185), SAPK/JNK, phospho-c-Jun (Ser73), c-Jun, Rel-B, NF-κB p65, 322 β-Actin and histone H3 were purchased from CST company.

    Article Title: Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.
    Article Snippet: This is a PDF file of an unedited peer-reviewed manuscript that has been accepted for publication.. NPG are providing this early version of the manuscript as a service to our customers.. The manuscript will undergo copyediting, typesetting and a proof review before it is published in its final form.

    Article Title: Role of MSC-derived Galectin 3 in the AML Microenvironment
    Article Snippet: STAT1 , stat1 , Cell Signaling , 9172 , Rabbit , 250.

    Article Title: Antagonism between viral infection and innate immunity at the single-cell level
    Article Snippet: STAT1 was reported to promote cell death [Kim & Lee, 2007, Cell Signal., doi:10.1016/j.cellsig.2006.09.003], so inhibition of STAT activity in infected cells is expected to promote virus spread.

    Article Title: The good and the bad faces of STAT1 in solid tumours.
    Article Snippet: Lee, STAT1 as a key modulator of cell death, Cell Signal 19 (2007) 454–465.

    Article Title: Interferon activates promoter of Nmi gene via interferon regulator factor-1.
    Article Snippet: N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses.. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated.. We identified and cloned the promoter of Nmi gene.

    Lactate Dehydrogenase Assay:


    Article Title: Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo
    Article Snippet: The phosphorylation status of STAT1 in cancer cells was assessed by treating the cells with 1,000 U of IFN-b for 24 h. The cell lysate collected from IFN-b-challenged and control-media-treated cells were subjected to immunoblotting to assess the levels of STAT1 (cat. no. 14994) and p-STAT1 (Tyr701, cat. no. 9167S) (Cell Signaling Technology).

    Article Title: Phosphatase wild-type p53-induced phosphatase 1 controls the development of T H 9 cells and allergic airway inflammation.
    Article Snippet: Phospho-STAT6 (Tyr641), STAT6, phospho-Smad3 319 (Ser423/425), Smad3, phospho-p38 MAPK (Thr180/Tyr182), P38, p-STAT5 320 (Tyr694), STAT5, p-STAT1 (Ser727), STAT1, phospho-SAPK/JNK 321 (Thr183/Tyr185), SAPK/JNK, phospho-c-Jun (Ser73), c-Jun, Rel-B, NF-κB p65, 322 β-Actin and histone H3 were purchased from CST company.

    Article Title: Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.
    Article Snippet: This is a PDF file of an unedited peer-reviewed manuscript that has been accepted for publication.. NPG are providing this early version of the manuscript as a service to our customers.. The manuscript will undergo copyediting, typesetting and a proof review before it is published in its final form.

    Article Title: Role of MSC-derived Galectin 3 in the AML Microenvironment
    Article Snippet: STAT1 , stat1 , Cell Signaling , 9172 , Rabbit , 250.

    Article Title: Antagonism between viral infection and innate immunity at the single-cell level
    Article Snippet: STAT1 was reported to promote cell death [Kim & Lee, 2007, Cell Signal., doi:10.1016/j.cellsig.2006.09.003], so inhibition of STAT activity in infected cells is expected to promote virus spread.

    Article Title: The good and the bad faces of STAT1 in solid tumours.
    Article Snippet: Lee, STAT1 as a key modulator of cell death, Cell Signal 19 (2007) 454–465.

    Article Title: Interferon activates promoter of Nmi gene via interferon regulator factor-1.
    Article Snippet: N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses.. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated.. We identified and cloned the promoter of Nmi gene.

    cDNA Synthesis:


    Article Title: Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo
    Article Snippet: The phosphorylation status of STAT1 in cancer cells was assessed by treating the cells with 1,000 U of IFN-b for 24 h. The cell lysate collected from IFN-b-challenged and control-media-treated cells were subjected to immunoblotting to assess the levels of STAT1 (cat. no. 14994) and p-STAT1 (Tyr701, cat. no. 9167S) (Cell Signaling Technology).

    Article Title: Phosphatase wild-type p53-induced phosphatase 1 controls the development of T H 9 cells and allergic airway inflammation.
    Article Snippet: Phospho-STAT6 (Tyr641), STAT6, phospho-Smad3 319 (Ser423/425), Smad3, phospho-p38 MAPK (Thr180/Tyr182), P38, p-STAT5 320 (Tyr694), STAT5, p-STAT1 (Ser727), STAT1, phospho-SAPK/JNK 321 (Thr183/Tyr185), SAPK/JNK, phospho-c-Jun (Ser73), c-Jun, Rel-B, NF-κB p65, 322 β-Actin and histone H3 were purchased from CST company.

    Article Title: Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.
    Article Snippet: This is a PDF file of an unedited peer-reviewed manuscript that has been accepted for publication.. NPG are providing this early version of the manuscript as a service to our customers.. The manuscript will undergo copyediting, typesetting and a proof review before it is published in its final form.

    Article Title: Role of MSC-derived Galectin 3 in the AML Microenvironment
    Article Snippet: STAT1 , stat1 , Cell Signaling , 9172 , Rabbit , 250.

    Article Title: Antagonism between viral infection and innate immunity at the single-cell level
    Article Snippet: STAT1 was reported to promote cell death [Kim & Lee, 2007, Cell Signal., doi:10.1016/j.cellsig.2006.09.003], so inhibition of STAT activity in infected cells is expected to promote virus spread.

    Article Title: The good and the bad faces of STAT1 in solid tumours.
    Article Snippet: Lee, STAT1 as a key modulator of cell death, Cell Signal 19 (2007) 454–465.

    Article Title: Interferon activates promoter of Nmi gene via interferon regulator factor-1.
    Article Snippet: N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses.. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated.. We identified and cloned the promoter of Nmi gene.

    Enzyme-linked Immunosorbent Assay:


    Article Title: Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo
    Article Snippet: The phosphorylation status of STAT1 in cancer cells was assessed by treating the cells with 1,000 U of IFN-b for 24 h. The cell lysate collected from IFN-b-challenged and control-media-treated cells were subjected to immunoblotting to assess the levels of STAT1 (cat. no. 14994) and p-STAT1 (Tyr701, cat. no. 9167S) (Cell Signaling Technology).

    Article Title: Phosphatase wild-type p53-induced phosphatase 1 controls the development of T H 9 cells and allergic airway inflammation.
    Article Snippet: Phospho-STAT6 (Tyr641), STAT6, phospho-Smad3 319 (Ser423/425), Smad3, phospho-p38 MAPK (Thr180/Tyr182), P38, p-STAT5 320 (Tyr694), STAT5, p-STAT1 (Ser727), STAT1, phospho-SAPK/JNK 321 (Thr183/Tyr185), SAPK/JNK, phospho-c-Jun (Ser73), c-Jun, Rel-B, NF-κB p65, 322 β-Actin and histone H3 were purchased from CST company.

    Article Title: Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.
    Article Snippet: This is a PDF file of an unedited peer-reviewed manuscript that has been accepted for publication.. NPG are providing this early version of the manuscript as a service to our customers.. The manuscript will undergo copyediting, typesetting and a proof review before it is published in its final form.

    Article Title: Role of MSC-derived Galectin 3 in the AML Microenvironment
    Article Snippet: STAT1 , stat1 , Cell Signaling , 9172 , Rabbit , 250.

    Article Title: Antagonism between viral infection and innate immunity at the single-cell level
    Article Snippet: STAT1 was reported to promote cell death [Kim & Lee, 2007, Cell Signal., doi:10.1016/j.cellsig.2006.09.003], so inhibition of STAT activity in infected cells is expected to promote virus spread.

    Article Title: The good and the bad faces of STAT1 in solid tumours.
    Article Snippet: Lee, STAT1 as a key modulator of cell death, Cell Signal 19 (2007) 454–465.

    Article Title: Interferon activates promoter of Nmi gene via interferon regulator factor-1.
    Article Snippet: N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses.. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated.. We identified and cloned the promoter of Nmi gene.

    Knock-Out:


    Article Title: Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo
    Article Snippet: The phosphorylation status of STAT1 in cancer cells was assessed by treating the cells with 1,000 U of IFN-b for 24 h. The cell lysate collected from IFN-b-challenged and control-media-treated cells were subjected to immunoblotting to assess the levels of STAT1 (cat. no. 14994) and p-STAT1 (Tyr701, cat. no. 9167S) (Cell Signaling Technology).

    Article Title: Phosphatase wild-type p53-induced phosphatase 1 controls the development of T H 9 cells and allergic airway inflammation.
    Article Snippet: Phospho-STAT6 (Tyr641), STAT6, phospho-Smad3 319 (Ser423/425), Smad3, phospho-p38 MAPK (Thr180/Tyr182), P38, p-STAT5 320 (Tyr694), STAT5, p-STAT1 (Ser727), STAT1, phospho-SAPK/JNK 321 (Thr183/Tyr185), SAPK/JNK, phospho-c-Jun (Ser73), c-Jun, Rel-B, NF-κB p65, 322 β-Actin and histone H3 were purchased from CST company.

    Article Title: Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.
    Article Snippet: This is a PDF file of an unedited peer-reviewed manuscript that has been accepted for publication.. NPG are providing this early version of the manuscript as a service to our customers.. The manuscript will undergo copyediting, typesetting and a proof review before it is published in its final form.

    Article Title: Role of MSC-derived Galectin 3 in the AML Microenvironment
    Article Snippet: STAT1 , stat1 , Cell Signaling , 9172 , Rabbit , 250.

    Article Title: Antagonism between viral infection and innate immunity at the single-cell level
    Article Snippet: STAT1 was reported to promote cell death [Kim & Lee, 2007, Cell Signal., doi:10.1016/j.cellsig.2006.09.003], so inhibition of STAT activity in infected cells is expected to promote virus spread.

    Article Title: The good and the bad faces of STAT1 in solid tumours.
    Article Snippet: Lee, STAT1 as a key modulator of cell death, Cell Signal 19 (2007) 454–465.

    Article Title: Interferon activates promoter of Nmi gene via interferon regulator factor-1.
    Article Snippet: N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses.. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated.. We identified and cloned the promoter of Nmi gene.

    Software:


    Article Title: Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo
    Article Snippet: The phosphorylation status of STAT1 in cancer cells was assessed by treating the cells with 1,000 U of IFN-b for 24 h. The cell lysate collected from IFN-b-challenged and control-media-treated cells were subjected to immunoblotting to assess the levels of STAT1 (cat. no. 14994) and p-STAT1 (Tyr701, cat. no. 9167S) (Cell Signaling Technology).

    Article Title: Phosphatase wild-type p53-induced phosphatase 1 controls the development of T H 9 cells and allergic airway inflammation.
    Article Snippet: Phospho-STAT6 (Tyr641), STAT6, phospho-Smad3 319 (Ser423/425), Smad3, phospho-p38 MAPK (Thr180/Tyr182), P38, p-STAT5 320 (Tyr694), STAT5, p-STAT1 (Ser727), STAT1, phospho-SAPK/JNK 321 (Thr183/Tyr185), SAPK/JNK, phospho-c-Jun (Ser73), c-Jun, Rel-B, NF-κB p65, 322 β-Actin and histone H3 were purchased from CST company.

    Article Title: Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.
    Article Snippet: This is a PDF file of an unedited peer-reviewed manuscript that has been accepted for publication.. NPG are providing this early version of the manuscript as a service to our customers.. The manuscript will undergo copyediting, typesetting and a proof review before it is published in its final form.

    Article Title: Role of MSC-derived Galectin 3 in the AML Microenvironment
    Article Snippet: STAT1 , stat1 , Cell Signaling , 9172 , Rabbit , 250.

    Article Title: Antagonism between viral infection and innate immunity at the single-cell level
    Article Snippet: STAT1 was reported to promote cell death [Kim & Lee, 2007, Cell Signal., doi:10.1016/j.cellsig.2006.09.003], so inhibition of STAT activity in infected cells is expected to promote virus spread.

    Article Title: The good and the bad faces of STAT1 in solid tumours.
    Article Snippet: Lee, STAT1 as a key modulator of cell death, Cell Signal 19 (2007) 454–465.

    Article Title: Interferon activates promoter of Nmi gene via interferon regulator factor-1.
    Article Snippet: N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses.. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated.. We identified and cloned the promoter of Nmi gene.

    Phospho-proteomics:


    Article Title: Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo
    Article Snippet: The phosphorylation status of STAT1 in cancer cells was assessed by treating the cells with 1,000 U of IFN-b for 24 h. The cell lysate collected from IFN-b-challenged and control-media-treated cells were subjected to immunoblotting to assess the levels of STAT1 (cat. no. 14994) and p-STAT1 (Tyr701, cat. no. 9167S) (Cell Signaling Technology).

    Article Title: Phosphatase wild-type p53-induced phosphatase 1 controls the development of T H 9 cells and allergic airway inflammation.
    Article Snippet: Phospho-STAT6 (Tyr641), STAT6, phospho-Smad3 319 (Ser423/425), Smad3, phospho-p38 MAPK (Thr180/Tyr182), P38, p-STAT5 320 (Tyr694), STAT5, p-STAT1 (Ser727), STAT1, phospho-SAPK/JNK 321 (Thr183/Tyr185), SAPK/JNK, phospho-c-Jun (Ser73), c-Jun, Rel-B, NF-κB p65, 322 β-Actin and histone H3 were purchased from CST company.

    Article Title: Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.
    Article Snippet: This is a PDF file of an unedited peer-reviewed manuscript that has been accepted for publication.. NPG are providing this early version of the manuscript as a service to our customers.. The manuscript will undergo copyediting, typesetting and a proof review before it is published in its final form.

    Article Title: Role of MSC-derived Galectin 3 in the AML Microenvironment
    Article Snippet: STAT1 , stat1 , Cell Signaling , 9172 , Rabbit , 250.

    Article Title: Antagonism between viral infection and innate immunity at the single-cell level
    Article Snippet: STAT1 was reported to promote cell death [Kim & Lee, 2007, Cell Signal., doi:10.1016/j.cellsig.2006.09.003], so inhibition of STAT activity in infected cells is expected to promote virus spread.

    Article Title: The good and the bad faces of STAT1 in solid tumours.
    Article Snippet: Lee, STAT1 as a key modulator of cell death, Cell Signal 19 (2007) 454–465.

    Article Title: Interferon activates promoter of Nmi gene via interferon regulator factor-1.
    Article Snippet: N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses.. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated.. We identified and cloned the promoter of Nmi gene.

    Control:


    Article Title: Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo
    Article Snippet: The phosphorylation status of STAT1 in cancer cells was assessed by treating the cells with 1,000 U of IFN-b for 24 h. The cell lysate collected from IFN-b-challenged and control-media-treated cells were subjected to immunoblotting to assess the levels of STAT1 (cat. no. 14994) and p-STAT1 (Tyr701, cat. no. 9167S) (Cell Signaling Technology).

    Article Title: Phosphatase wild-type p53-induced phosphatase 1 controls the development of T H 9 cells and allergic airway inflammation.
    Article Snippet: Phospho-STAT6 (Tyr641), STAT6, phospho-Smad3 319 (Ser423/425), Smad3, phospho-p38 MAPK (Thr180/Tyr182), P38, p-STAT5 320 (Tyr694), STAT5, p-STAT1 (Ser727), STAT1, phospho-SAPK/JNK 321 (Thr183/Tyr185), SAPK/JNK, phospho-c-Jun (Ser73), c-Jun, Rel-B, NF-κB p65, 322 β-Actin and histone H3 were purchased from CST company.

    Article Title: Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.
    Article Snippet: This is a PDF file of an unedited peer-reviewed manuscript that has been accepted for publication.. NPG are providing this early version of the manuscript as a service to our customers.. The manuscript will undergo copyediting, typesetting and a proof review before it is published in its final form.

    Article Title: Role of MSC-derived Galectin 3 in the AML Microenvironment
    Article Snippet: STAT1 , stat1 , Cell Signaling , 9172 , Rabbit , 250.

    Article Title: Antagonism between viral infection and innate immunity at the single-cell level
    Article Snippet: STAT1 was reported to promote cell death [Kim & Lee, 2007, Cell Signal., doi:10.1016/j.cellsig.2006.09.003], so inhibition of STAT activity in infected cells is expected to promote virus spread.

    Article Title: The good and the bad faces of STAT1 in solid tumours.
    Article Snippet: Lee, STAT1 as a key modulator of cell death, Cell Signal 19 (2007) 454–465.

    Article Title: Interferon activates promoter of Nmi gene via interferon regulator factor-1.
    Article Snippet: N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses.. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated.. We identified and cloned the promoter of Nmi gene.

    Transduction:


    Article Title: Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo
    Article Snippet: The phosphorylation status of STAT1 in cancer cells was assessed by treating the cells with 1,000 U of IFN-b for 24 h. The cell lysate collected from IFN-b-challenged and control-media-treated cells were subjected to immunoblotting to assess the levels of STAT1 (cat. no. 14994) and p-STAT1 (Tyr701, cat. no. 9167S) (Cell Signaling Technology).

    Article Title: Phosphatase wild-type p53-induced phosphatase 1 controls the development of T H 9 cells and allergic airway inflammation.
    Article Snippet: Phospho-STAT6 (Tyr641), STAT6, phospho-Smad3 319 (Ser423/425), Smad3, phospho-p38 MAPK (Thr180/Tyr182), P38, p-STAT5 320 (Tyr694), STAT5, p-STAT1 (Ser727), STAT1, phospho-SAPK/JNK 321 (Thr183/Tyr185), SAPK/JNK, phospho-c-Jun (Ser73), c-Jun, Rel-B, NF-κB p65, 322 β-Actin and histone H3 were purchased from CST company.

    Article Title: Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.
    Article Snippet: This is a PDF file of an unedited peer-reviewed manuscript that has been accepted for publication.. NPG are providing this early version of the manuscript as a service to our customers.. The manuscript will undergo copyediting, typesetting and a proof review before it is published in its final form.

    Article Title: Role of MSC-derived Galectin 3 in the AML Microenvironment
    Article Snippet: STAT1 , stat1 , Cell Signaling , 9172 , Rabbit , 250.

    Article Title: Antagonism between viral infection and innate immunity at the single-cell level
    Article Snippet: STAT1 was reported to promote cell death [Kim & Lee, 2007, Cell Signal., doi:10.1016/j.cellsig.2006.09.003], so inhibition of STAT activity in infected cells is expected to promote virus spread.

    Article Title: The good and the bad faces of STAT1 in solid tumours.
    Article Snippet: Lee, STAT1 as a key modulator of cell death, Cell Signal 19 (2007) 454–465.

    Article Title: Interferon activates promoter of Nmi gene via interferon regulator factor-1.
    Article Snippet: N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses.. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated.. We identified and cloned the promoter of Nmi gene.

    shRNA:


    Article Title: Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo
    Article Snippet: The phosphorylation status of STAT1 in cancer cells was assessed by treating the cells with 1,000 U of IFN-b for 24 h. The cell lysate collected from IFN-b-challenged and control-media-treated cells were subjected to immunoblotting to assess the levels of STAT1 (cat. no. 14994) and p-STAT1 (Tyr701, cat. no. 9167S) (Cell Signaling Technology).

    Article Title: Phosphatase wild-type p53-induced phosphatase 1 controls the development of T H 9 cells and allergic airway inflammation.
    Article Snippet: Phospho-STAT6 (Tyr641), STAT6, phospho-Smad3 319 (Ser423/425), Smad3, phospho-p38 MAPK (Thr180/Tyr182), P38, p-STAT5 320 (Tyr694), STAT5, p-STAT1 (Ser727), STAT1, phospho-SAPK/JNK 321 (Thr183/Tyr185), SAPK/JNK, phospho-c-Jun (Ser73), c-Jun, Rel-B, NF-κB p65, 322 β-Actin and histone H3 were purchased from CST company.

    Article Title: Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.
    Article Snippet: This is a PDF file of an unedited peer-reviewed manuscript that has been accepted for publication.. NPG are providing this early version of the manuscript as a service to our customers.. The manuscript will undergo copyediting, typesetting and a proof review before it is published in its final form.

    Article Title: Role of MSC-derived Galectin 3 in the AML Microenvironment
    Article Snippet: STAT1 , stat1 , Cell Signaling , 9172 , Rabbit , 250.

    Article Title: Antagonism between viral infection and innate immunity at the single-cell level
    Article Snippet: STAT1 was reported to promote cell death [Kim & Lee, 2007, Cell Signal., doi:10.1016/j.cellsig.2006.09.003], so inhibition of STAT activity in infected cells is expected to promote virus spread.

    Article Title: The good and the bad faces of STAT1 in solid tumours.
    Article Snippet: Lee, STAT1 as a key modulator of cell death, Cell Signal 19 (2007) 454–465.

    Article Title: Interferon activates promoter of Nmi gene via interferon regulator factor-1.
    Article Snippet: N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses.. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated.. We identified and cloned the promoter of Nmi gene.

    Sequencing:


    Article Title: Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo
    Article Snippet: The phosphorylation status of STAT1 in cancer cells was assessed by treating the cells with 1,000 U of IFN-b for 24 h. The cell lysate collected from IFN-b-challenged and control-media-treated cells were subjected to immunoblotting to assess the levels of STAT1 (cat. no. 14994) and p-STAT1 (Tyr701, cat. no. 9167S) (Cell Signaling Technology).

    Article Title: Phosphatase wild-type p53-induced phosphatase 1 controls the development of T H 9 cells and allergic airway inflammation.
    Article Snippet: Phospho-STAT6 (Tyr641), STAT6, phospho-Smad3 319 (Ser423/425), Smad3, phospho-p38 MAPK (Thr180/Tyr182), P38, p-STAT5 320 (Tyr694), STAT5, p-STAT1 (Ser727), STAT1, phospho-SAPK/JNK 321 (Thr183/Tyr185), SAPK/JNK, phospho-c-Jun (Ser73), c-Jun, Rel-B, NF-κB p65, 322 β-Actin and histone H3 were purchased from CST company.

    Article Title: Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.
    Article Snippet: This is a PDF file of an unedited peer-reviewed manuscript that has been accepted for publication.. NPG are providing this early version of the manuscript as a service to our customers.. The manuscript will undergo copyediting, typesetting and a proof review before it is published in its final form.

    Article Title: Role of MSC-derived Galectin 3 in the AML Microenvironment
    Article Snippet: STAT1 , stat1 , Cell Signaling , 9172 , Rabbit , 250.

    Article Title: Antagonism between viral infection and innate immunity at the single-cell level
    Article Snippet: STAT1 was reported to promote cell death [Kim & Lee, 2007, Cell Signal., doi:10.1016/j.cellsig.2006.09.003], so inhibition of STAT activity in infected cells is expected to promote virus spread.

    Article Title: The good and the bad faces of STAT1 in solid tumours.
    Article Snippet: Lee, STAT1 as a key modulator of cell death, Cell Signal 19 (2007) 454–465.

    Article Title: Interferon activates promoter of Nmi gene via interferon regulator factor-1.
    Article Snippet: N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses.. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated.. We identified and cloned the promoter of Nmi gene.

    Clone Assay:


    Article Title: Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo
    Article Snippet: The phosphorylation status of STAT1 in cancer cells was assessed by treating the cells with 1,000 U of IFN-b for 24 h. The cell lysate collected from IFN-b-challenged and control-media-treated cells were subjected to immunoblotting to assess the levels of STAT1 (cat. no. 14994) and p-STAT1 (Tyr701, cat. no. 9167S) (Cell Signaling Technology).

    Article Title: Phosphatase wild-type p53-induced phosphatase 1 controls the development of T H 9 cells and allergic airway inflammation.
    Article Snippet: Phospho-STAT6 (Tyr641), STAT6, phospho-Smad3 319 (Ser423/425), Smad3, phospho-p38 MAPK (Thr180/Tyr182), P38, p-STAT5 320 (Tyr694), STAT5, p-STAT1 (Ser727), STAT1, phospho-SAPK/JNK 321 (Thr183/Tyr185), SAPK/JNK, phospho-c-Jun (Ser73), c-Jun, Rel-B, NF-κB p65, 322 β-Actin and histone H3 were purchased from CST company.

    Article Title: Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.
    Article Snippet: This is a PDF file of an unedited peer-reviewed manuscript that has been accepted for publication.. NPG are providing this early version of the manuscript as a service to our customers.. The manuscript will undergo copyediting, typesetting and a proof review before it is published in its final form.

    Article Title: Role of MSC-derived Galectin 3 in the AML Microenvironment
    Article Snippet: STAT1 , stat1 , Cell Signaling , 9172 , Rabbit , 250.

    Article Title: Antagonism between viral infection and innate immunity at the single-cell level
    Article Snippet: STAT1 was reported to promote cell death [Kim & Lee, 2007, Cell Signal., doi:10.1016/j.cellsig.2006.09.003], so inhibition of STAT activity in infected cells is expected to promote virus spread.

    Article Title: The good and the bad faces of STAT1 in solid tumours.
    Article Snippet: Lee, STAT1 as a key modulator of cell death, Cell Signal 19 (2007) 454–465.

    Article Title: Interferon activates promoter of Nmi gene via interferon regulator factor-1.
    Article Snippet: N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses.. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated.. We identified and cloned the promoter of Nmi gene.

    Plasmid Preparation:


    Article Title: Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo
    Article Snippet: The phosphorylation status of STAT1 in cancer cells was assessed by treating the cells with 1,000 U of IFN-b for 24 h. The cell lysate collected from IFN-b-challenged and control-media-treated cells were subjected to immunoblotting to assess the levels of STAT1 (cat. no. 14994) and p-STAT1 (Tyr701, cat. no. 9167S) (Cell Signaling Technology).

    Article Title: Phosphatase wild-type p53-induced phosphatase 1 controls the development of T H 9 cells and allergic airway inflammation.
    Article Snippet: Phospho-STAT6 (Tyr641), STAT6, phospho-Smad3 319 (Ser423/425), Smad3, phospho-p38 MAPK (Thr180/Tyr182), P38, p-STAT5 320 (Tyr694), STAT5, p-STAT1 (Ser727), STAT1, phospho-SAPK/JNK 321 (Thr183/Tyr185), SAPK/JNK, phospho-c-Jun (Ser73), c-Jun, Rel-B, NF-κB p65, 322 β-Actin and histone H3 were purchased from CST company.

    Article Title: Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.
    Article Snippet: This is a PDF file of an unedited peer-reviewed manuscript that has been accepted for publication.. NPG are providing this early version of the manuscript as a service to our customers.. The manuscript will undergo copyediting, typesetting and a proof review before it is published in its final form.

    Article Title: Role of MSC-derived Galectin 3 in the AML Microenvironment
    Article Snippet: STAT1 , stat1 , Cell Signaling , 9172 , Rabbit , 250.

    Article Title: Antagonism between viral infection and innate immunity at the single-cell level
    Article Snippet: STAT1 was reported to promote cell death [Kim & Lee, 2007, Cell Signal., doi:10.1016/j.cellsig.2006.09.003], so inhibition of STAT activity in infected cells is expected to promote virus spread.

    Article Title: The good and the bad faces of STAT1 in solid tumours.
    Article Snippet: Lee, STAT1 as a key modulator of cell death, Cell Signal 19 (2007) 454–465.

    Article Title: Interferon activates promoter of Nmi gene via interferon regulator factor-1.
    Article Snippet: N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses.. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated.. We identified and cloned the promoter of Nmi gene.



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    Hyperosmolarity induced DED-associated injury and inflammation of conjunctival organoids. ( A ) Representative images showing hyperosmolarity-induced organoid disintegration ( scale bar , 100 µm). ( B ) Quantification of the percentage of intact organoids. ( C ) Western blotting analysis of proliferation markers (p63 and Ki67), inflammatory mediators (MMP-9 and IL-1β), signaling molecules (NF-κB p65, STAT1, and PI3K), ferroptosis markers (TFR and GPX4), and apoptosis marker (caspase-3). ( D – F ) Immunofluorescence staining of p63 + and Ki67 + cells in organoids (scale bar , 50 µm). ( G ) Immunofluorescence staining of caspase-9, NF-κB p65, STAT1, and IL-1β in organoids ( scale bar , 50 µm). * P < 0.05; *** P < 0.001; **** P < 0.0001; ns, not significant.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Ly93 Inhibits Sphingomyelin Synthesis and Attenuates Inflammation and Injury in Dry Eye Conjunctival Organoids

    doi: 10.1167/iovs.67.2.58

    Figure Lengend Snippet: Hyperosmolarity induced DED-associated injury and inflammation of conjunctival organoids. ( A ) Representative images showing hyperosmolarity-induced organoid disintegration ( scale bar , 100 µm). ( B ) Quantification of the percentage of intact organoids. ( C ) Western blotting analysis of proliferation markers (p63 and Ki67), inflammatory mediators (MMP-9 and IL-1β), signaling molecules (NF-κB p65, STAT1, and PI3K), ferroptosis markers (TFR and GPX4), and apoptosis marker (caspase-3). ( D – F ) Immunofluorescence staining of p63 + and Ki67 + cells in organoids (scale bar , 50 µm). ( G ) Immunofluorescence staining of caspase-9, NF-κB p65, STAT1, and IL-1β in organoids ( scale bar , 50 µm). * P < 0.05; *** P < 0.001; **** P < 0.0001; ns, not significant.

    Article Snippet: Immunofluorescence staining was performed to evaluate the expression of K19 (1:200, Cell Signaling Technology, Danvers, MA, USA), K13 (1:200, Cell Signaling Technology), IL-1β (1:200, Abcam, Cambridge, UK), STAT1 (1:200, Proteintech, Rosemont, IL, USA), p63 (1:250, Abcam), Ki67 (1:200, Abcam), NF-κB p65 (1:200, Abcam), MUC1 (1:200, Abcam), MUC5AC(1:250, Abcam), caspase-9 (1:200, Cell Signaling Technology), ZO-1 (1:200, Abcam), and 4-hydroxynonenal (4-HNE) (1:100, Invitrogen, Carlsbad, CA, USA) in frozen sections of the conjunctival organoids.

    Techniques: Western Blot, Marker, Immunofluorescence, Staining

    Exogenous SM induced inflammation and injury of conjunctival organoids. ( A ) Effects of SM treatment on the viability of conjunctival cells and morphology of organoids ( scale bar , 100 µm for cells and 200 µm for organoids). ( B , C ) Western blotting analysis of inflammatory markers (phospho-STAT1, STAT1, IL-1β, and iNOS) in SM-treated organoids. ( D – F ) Relative mRNA expression of IL-1β, IL-6, and STAT1 after SM treatment. ( G ) Immunofluorescence staining of K13, K19, Ki67, MUC1, and MUC5AC in SM-treated organoids ( scale bar , 50 µm). ( H – L ) Comparative analysis of immunofluorescent results: K13 ( H ), K19 ( I ), Ki67 ( J ), MUC1 ( K ), and MUC5AC ( L ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, not significant.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Ly93 Inhibits Sphingomyelin Synthesis and Attenuates Inflammation and Injury in Dry Eye Conjunctival Organoids

    doi: 10.1167/iovs.67.2.58

    Figure Lengend Snippet: Exogenous SM induced inflammation and injury of conjunctival organoids. ( A ) Effects of SM treatment on the viability of conjunctival cells and morphology of organoids ( scale bar , 100 µm for cells and 200 µm for organoids). ( B , C ) Western blotting analysis of inflammatory markers (phospho-STAT1, STAT1, IL-1β, and iNOS) in SM-treated organoids. ( D – F ) Relative mRNA expression of IL-1β, IL-6, and STAT1 after SM treatment. ( G ) Immunofluorescence staining of K13, K19, Ki67, MUC1, and MUC5AC in SM-treated organoids ( scale bar , 50 µm). ( H – L ) Comparative analysis of immunofluorescent results: K13 ( H ), K19 ( I ), Ki67 ( J ), MUC1 ( K ), and MUC5AC ( L ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, not significant.

    Article Snippet: Immunofluorescence staining was performed to evaluate the expression of K19 (1:200, Cell Signaling Technology, Danvers, MA, USA), K13 (1:200, Cell Signaling Technology), IL-1β (1:200, Abcam, Cambridge, UK), STAT1 (1:200, Proteintech, Rosemont, IL, USA), p63 (1:250, Abcam), Ki67 (1:200, Abcam), NF-κB p65 (1:200, Abcam), MUC1 (1:200, Abcam), MUC5AC(1:250, Abcam), caspase-9 (1:200, Cell Signaling Technology), ZO-1 (1:200, Abcam), and 4-hydroxynonenal (4-HNE) (1:100, Invitrogen, Carlsbad, CA, USA) in frozen sections of the conjunctival organoids.

    Techniques: Western Blot, Expressing, Immunofluorescence, Staining

    Ly93 attenuated DED-associated inflammation in conjunctival organoids. ( A ) Immunofluorescence staining of caspase-9, ZO-1, IL-1β, STAT1, and NF-κB p65 between vehicle control, DED, and DED + Ly93 groups ( scale bar , 50 µm). ( B ) Intracellular Fe 2+ analysis ( scale bar , 50 µm). ( C ) Immunofluorescence staining of 4-HNE ( scale bar , 50 µm). ( D – H ) Western blotting analysis of GPX4 ( D ), TFR ( E ), AKT pathway ( F ), caspase-9 and NF-κB p65 pathway ( G ), and IL-1β and STAT1 pathway ( H ) protein expression.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Ly93 Inhibits Sphingomyelin Synthesis and Attenuates Inflammation and Injury in Dry Eye Conjunctival Organoids

    doi: 10.1167/iovs.67.2.58

    Figure Lengend Snippet: Ly93 attenuated DED-associated inflammation in conjunctival organoids. ( A ) Immunofluorescence staining of caspase-9, ZO-1, IL-1β, STAT1, and NF-κB p65 between vehicle control, DED, and DED + Ly93 groups ( scale bar , 50 µm). ( B ) Intracellular Fe 2+ analysis ( scale bar , 50 µm). ( C ) Immunofluorescence staining of 4-HNE ( scale bar , 50 µm). ( D – H ) Western blotting analysis of GPX4 ( D ), TFR ( E ), AKT pathway ( F ), caspase-9 and NF-κB p65 pathway ( G ), and IL-1β and STAT1 pathway ( H ) protein expression.

    Article Snippet: Immunofluorescence staining was performed to evaluate the expression of K19 (1:200, Cell Signaling Technology, Danvers, MA, USA), K13 (1:200, Cell Signaling Technology), IL-1β (1:200, Abcam, Cambridge, UK), STAT1 (1:200, Proteintech, Rosemont, IL, USA), p63 (1:250, Abcam), Ki67 (1:200, Abcam), NF-κB p65 (1:200, Abcam), MUC1 (1:200, Abcam), MUC5AC(1:250, Abcam), caspase-9 (1:200, Cell Signaling Technology), ZO-1 (1:200, Abcam), and 4-hydroxynonenal (4-HNE) (1:100, Invitrogen, Carlsbad, CA, USA) in frozen sections of the conjunctival organoids.

    Techniques: Immunofluorescence, Staining, Control, Western Blot, Expressing

    Rapamycin co‐treatment mitigates proinflammatory transcriptional hyperactivation in aged mice exposed to HFD. (A) Protocol: Mice received ND or eRapamycin‐containing diet from 4 to 18 months, followed by 9 weeks on HFD or ND. (B) Western blot of liver tissue. (C) Fold change of p‐S6/S6 compared to control diet. (D) PCA of RNA‐seq from isolated hepatocytes. (E) Number of DEGs across treatment groups. (F) Scatter plot comparing HFD‐induced gene expression changes (HFD + veh vs. ND + veh) to all gene expression changes with eRapamycin (eRapa+HFD vs. HFD + veh). (G) Heatmap of genes upregulated by HFD + vehicle treatment. (H) Venn diagram showing overlap between transcripts elevated by HFD versus ND and those decreased by rapamycin. (I) KEGG enrichment analysis of overlapping genes. (J) Heatmap of innate immune response genes. (K) Top transcription factor pathways predicted by IPA in the overlapping gene set. (L) Heatmap of Stat1 target genes. (M) Tumorigenic index score calculated per mouse. Statistical analyses for p‐S6/S6 ratios and tumorigenic index scores were performed using one‐way ANOVA with post hoc Tukey's test; p < 0.05 (*), p < 0.1 (**).

    Journal: Aging Cell

    Article Title: Rapamycin Reverses the Hepatic Response to Diet‐Induced Metabolic Stress That Is Amplified by Aging

    doi: 10.1111/acel.70395

    Figure Lengend Snippet: Rapamycin co‐treatment mitigates proinflammatory transcriptional hyperactivation in aged mice exposed to HFD. (A) Protocol: Mice received ND or eRapamycin‐containing diet from 4 to 18 months, followed by 9 weeks on HFD or ND. (B) Western blot of liver tissue. (C) Fold change of p‐S6/S6 compared to control diet. (D) PCA of RNA‐seq from isolated hepatocytes. (E) Number of DEGs across treatment groups. (F) Scatter plot comparing HFD‐induced gene expression changes (HFD + veh vs. ND + veh) to all gene expression changes with eRapamycin (eRapa+HFD vs. HFD + veh). (G) Heatmap of genes upregulated by HFD + vehicle treatment. (H) Venn diagram showing overlap between transcripts elevated by HFD versus ND and those decreased by rapamycin. (I) KEGG enrichment analysis of overlapping genes. (J) Heatmap of innate immune response genes. (K) Top transcription factor pathways predicted by IPA in the overlapping gene set. (L) Heatmap of Stat1 target genes. (M) Tumorigenic index score calculated per mouse. Statistical analyses for p‐S6/S6 ratios and tumorigenic index scores were performed using one‐way ANOVA with post hoc Tukey's test; p < 0.05 (*), p < 0.1 (**).

    Article Snippet: Antibodies used: total Stat1 (Cell Signaling Technologies 9172S), S6 Ribosomal protein (Cell Signaling Technologies 2317), phospho‐S6 Ribosomal protein (Cell Signaling Technologies 5364).

    Techniques: Western Blot, Control, RNA Sequencing, Isolation, Gene Expression